Unit 1 · Basics in cell culture (Weeks 1 to 5)
Lab Week 1: Lab Intro, Pipetting and Calculations
Lab safety, waste disposal, pipetting and the lab math she drills on every Concept Quiz.
Unit 2 · Cellular assays (Weeks 6 to 8)
Lab Week 7: Week 7 Experiments
Cell proliferation experiment and the toxicity by trypan blue setup.
Lab Week 8: Week 8 Experiment (Alamar Blue)
Pointers for the day, TX-100 toxicity by Alamar Blue, and the Alamar Blue redox readout.
Protocols and experiment handouts (Weeks 2 to 8)
Week 2 Aseptic Technique Protocol
Observing cells, aseptic technique in the hood and preparing media.
Week 4 Hemocytometry Protocol
Counting cells on the hemocytometer: loading, which squares to count, and the calculations.
Week 5 Cryopreservation Protocol
Lab 4 freezing experiment: freezing medium, cooling conditions, storage and thawing.
Week 6 Staining Protocol
Fixing, quenching, permeabilizing and staining cells with DAPI and Texas Red-phalloidin.
Week 7 Trypan Blue Protocol
Trypan blue viability: the two methods, materials, staining and counting.
Week 7 Experiment Handout
Cell split for the growth curve and the ethanol viability experiment, plate layout and analysis questions.
Week 8 Alamar Blue Protocol
Alamar Blue viability assay: materials, volumes, incubation and plate reader settings.
Week 8 TX-100 Experiment Handout
TX-100 toxicity by Alamar Blue: cell split, treatment, pointers and thought questions.